Monitoring at MacMillan Wharf, Provincetown, MA

Monitoring at MacMillan Wharf, Provincetown, MA
Marine Invasive Species (MIS) Monitoring at MacMillan Wharf, Provincetown, MA.

Tuesday, October 30, 2012

MIS Floating Dock Community

Ascidians Claiming Space

On my last visit to Provincetown, I obtained a nice photograph of an invertebrate marine floating dock community at MacMillan Wharf - a shaded section of a float that was facing north, under a dock overhang, that had ascidians and other invertebrates growing together.  The upper few inches lacked a border of algae along the waterline that is characteristic of sunny locations.  It was a scene of moderate growth, before the species had a chance to completely overgrow each other into a mass of organisms (an enlargment of the whole photo is showing in the footer at the bottom of the blog).

Community of Invertebrates Below the Water Line
Species that are easily identified in the photo or enlargements:
  • Styela clava, one large individual 8-10 cm long cloaked in Didemnum vexillum and several smaller individuals about 3-5 cm long growing along the waterline.
  • Didemnum vexillum - Beige areas on the float, growing over Styela and other species. Didemnum was less abundant in 2012 than 2011. This float was one location in the marina where Didemnum was well-established and living together with Diplosoma.
  • Diplosoma listerianum - Grey-colored, flat colonies that grow on the float and over Styela.  Diplosoma was the the most abundant ascidian in Provincetown in 2012, growing on many surfaces that were dominated by Didemnum last year.
  • Botrylloides violaceus - Various small colonies, some tiny, in at least 2 shades of orange. Present everywhere but usually in limited discrete colonies. 
  • Botryllus schlosseri - A single colony can be seen in the enlargement below. 
  • Bugula neritina - A single wine-red colony about 2 cm high in the left center, immediately below a light-orange Botrylloides.
  A single mosaic-patterned colony of Botryllus schlosseri (left bottom) and a row of 5 Styela individuals (right) line up along the water line.  The first 4 Styela have clean, brown tunics whereas the 5th one on the right is partly covered by Diplosoma.    
Palaemonid shrimp can also be seen in the photo with pairs of flash-induced "white-eye" of their normally black eyes. There are 5 pairs of eyes in the photo (1 on the left side of the large Styela and 4 lined-up on the right).  
This year, a few Palaemon elegans, an MIS shrimp with blue-banded legs that is spreading throughout the Gulf of Maine, were seen at MacMillan Wharf.

Sunday, October 28, 2012

Measuring Salinity of Seawater

Bring along the Refractometer

Seawater in the world's oceans has a salinity of about 35 parts per thousand.  Although the vast majority of seawater has a salinity of between 31 and 38 ppt, seawater is not uniformly saline throughout the world.  Where mixing occurs with fresh water runoff from the mouths of rivers or near melting glaciers, seawater can be substantially diluted. The most saline water is located at areas where high rates of evaporation and low levels of precipitation, river inflow, and circulation result in salty water (e.g. the Mediterranean and Red Seas).  Seawater is primarily composed of the positive ions Sodium (Na+), Magnesium (Mg+), Calcium (Ca++), and Potassium (K+), and the negative ions Chloride (Cl-) and Sulfate (SO4-2).  

Table of the Relative Amounts of Salts in Seawater

The salinity of seawater is determined with a refractometer, a laboratory or field device for the measurement of any aqueous solution using its index of refraction.  They are commonly used in science, medicine, brewing, and beverage production.  A different kind of refractometer is needed for each application, including seawater salinity.  In clinical medicine, a refractometer is used to measure protein concentration in human fluids such as urine, and in the food industry, a brix refractometer is used to measure the concentration of sugar in beverages.  In marine aquarium keeping, a refractometer is used to measure the salinity and specific gravity of aquarium water.   A seawater refractometer can be obtained online from a biological research laboratory supply company or retail stores that sell salt water aquarium supplies.

Hand-Held Refractometer
This refractometer measures salinity from 0 to 100 parts per thousand.  Left, held like a telescope, a sample of seawater is placed in the chamber and salinity is determined on a scale seen through the refractometer eyepiece.  Right, view through the eyepiece.  Salinity is expressed as optical density on the left scale and parts per thousand (ppt) on the right. 

Salinity in marinas on monitoring trips during summers of 2011-2012 ranged from 32-35 ppt in Provincetown and 30-34 pp in Wellfleet. These readings are not unexpected since the outer Cape Cod is continually replenished by currents in the Gulf of Maine and by high tides that penetrate up into streams.  The Cape also has a limited supply of fresh water from run-off and streams in comparison to Boston Harbor (Wellfleet Marina is at the mouth of a small stream, Duck Creek, and several streams empty into other areas of the harbor).   

Salinity in the Outer Cape

Holmes-Farley, R.  Refractometers and Salinity Measurement:  Reefkeeping: An online magazine for the marine aquarist. 
Wiki Refractometer
Seawater Refractometer Model IModel IIModel III 
ATC Natural Seawater Refractometer